AKTIVITAS ANTIOKSIDAN MADU HUTAN DI BEBERAPA KECAMATAN DI KABUPATEN SUMBAWA BESAR
DOI:
https://doi.org/10.36761/jt.v2i3.170Keywords:
madu hutan, antioksidan, SumbawaAbstract
Madu merupakan zat alami yang dihasilkan oleh lebah madu dari nektar bunga dan sekresi tanaman. Madu mengandung mineral, protein, vitamin, asam organik, flavonoid, senyawa fenolik, dan enzim seperti katalase, peroksida, glukosa oksidasi, dan fitokimia lainnya, madu juga memiliki aktivitas antioksidan enzimatik dan non-enzimatik. Komposisi utama madu terdiri dari 80% karbohidrat, 17-20% air, 1-2% mineral dan senyawa organik. Penelitian ini bertujuan untuk identifikasi kandungan antioksidan pada madu hutan di beberapa daerah di Kabupaten Sumbawa. Untuk identifikasi kandungan antioksidan dilakukan analisa aktivitas antioksidan, total fenol, ekstraksi senyawa fenolik, dan identifikasi senyawa fenolik pada madu. Jumlah sampel yang digunakan adalah sampel madu yang diperoleh dari 2 lokasi hutan di Kecamatan Batu Lanteh, 1 lokasi hutan Kecamatan Labuhan Badas dan 1 lokasi hutan Kecamatan Lape Kabupaten Sumbawa. Hasil dari penelitian menunjukkan bahwa madu hutan di beberapa daerah tersebut mengandung senyawa fenol (asam galat) sebesar 0,0633 – 0,3875 mg/g dan memiliki aktivitas antioksidan antara 3,3365 – 30,9680% dengan nilai IC50 60,2 – 572,3 mg/mL.
References
Darmawan, S. dan Agustarini, R., 2011. Penurunan Kadar Air Madu Hutan Alam Sumbawa. Prosiding Seminar Nasional Peranan Hasil Litbang HHBK dalam Mendukung Pembangunan Kehutanan: 313-321
Ferreira, I.C.F.R. et al., 2009. Antioxidant Activity of Portuguese Honey Samples: Different Contributions of the Entire Honey and Phenolic Extract. Food Chemistry Vol. 114 issue 4: 1438 -1443
Hussein, S.Z., Yusoff, K.M., Makpol, S., and Yusof, Y.A.M., 2011. Antioxidant Capacities and Total Phenolic Contents Increase with Gamma Irradiation in Two Types of Malaysian Honey. Molecules Vol. 16: 6378-6395
Khalil, M.I., Sulaiman, S.A., and Boukraa, L., 2010. Antioxidant Properties of Honey and its Role in Preventing Health Disorder. The Open Nutraceuticals Journal Vol. 3: 6-16
Khalil, MD.I. et al., 2012. Content and Antioxidant Properties of Processed Tualang Honey Collected From Different Regions in Malaysia. International Journal of Pharmacy and Pharmaceutical Sciences Vol. 4: 214 – 219.
Kumazawa, S. et al., 2012. Antioxidant Activity in Honeys of Various Floral Origins: Isolation and Identification of Antioxidants in Peppermint Honey. Food Science and Technology Research Vol. 18 (5): 679 – 685.
Lachman, J. et al., Contents of Major Phenolic and Flavonoid Antioxidants in Selected Czech Honey. Czech Journal of Food Science Vol. 28: 412 - 426
Marshall, S.M., 2013. Identification and Concentration of Phenolic and Carbonyl Compounds in Florida Honeys. Thesis-University of Florida: Florida
Moniruzzaman, M. et al., 2013. Physicochemical and Antioxidant Properties of Malaysian Honey Produced by Apis cerana, Apis dorsata and Apis melifera. BMC Complementary and Alternative Medicine Vol. 13: 1 - 12
Nagai, T. et al., 2012. Functional Property of Honey from Echium vulgare. Food and Nutrition Science Vol. 3: 614 – 620
Nascimento, D.L. et al., 2012. Extreme Effects of Season on The Foraging Activities and Colony Productivity of a Stingless Bee (Melipona asilvai Moure, 1971) in Northeast Brazil. Psyche: A Journal of Entomology Vol. 2012 artile ID 267361
Neupane, B.P. et al., 2015 Antioxidant Properties of Honey From Different Altitudes of Nepal Himalayas. Polish Journal of Food and Nutrition Science Vol. 65 No.2: 87 - 91
Pontis, J.A., Costa, M.A., Silva, S.J.R., and Flach, A., 2014. Color, Phenolic, Flavonoid Content and Antioxidant Activity of Honey from Roraima, Brazil. Food Science and Technology Vol. 34(1): 69-73
Prica, N. et al., 2014. Moisture and Acidity as Indicators of the Quality of Honey Originating From Vojvodina Region. Arhiv Veterinarske Medicine Vol. 7 No.2: 99 -109
Puskadija, Z. et al., 2007. Influence of Weather Conditions on Honey Bee Visits (Apis melifera carnica) During Sunflower (Helianthus annuus L.) Blooming Period. ISSN 1330-7142
Sime, D., Atlabachew, M., Abshiro, M. R., and Zewde, T., 2015. Total Phenols and Antioxidant Activities of Natural Honeys and Propolis Collected from Different Geographical Regions of Ethiopia. Bull Chem Soc Ethiop. 29 (2): 163-172.
Siti, N. dkk., 2016. Penetapan Kadar Vitamin C pada Jerami Nangka (Artocarpus hetertopophyllus L.). Jurnal Farmasi Sains dan Praktis Vol.II No1
Trimanto dan Sialiaan, F.A., 2017. Botanical Survey in Moyo Island, West Nusa Tenggara, Indonesia: Inventory of Flora Collection at Forest. The Journal of Tropical Life Science Vol. 7 No.2: 158 – 166
