ANALISIS KANDUNGAN ASAM ASKORBAT PADA TANAMAN KANGKUNG (Ipomoea reptana Poir), BAYAM (Amaranthus spinosus), dan KETIMUN (Cucumis sativus L)
DOI:
https://doi.org/10.36761/jt.v4i1.567Keywords:
Askorbat, kangkung, bayam, ketimunAbstract
Plants have a defense mechanism against increasing oxidative compounds formed due to drought stress. One of the compounds forming these compounds is ascorbic acid. Ascorbate is the main metabolite compound in plants that has an antioxidant function, which protects plants from oxidative damage resulting from aerobic metabolism. The aim of the study was to standardize and analyze the content of Ascorbate (ASA) on the leaves of kale (Ipomoea reptana Poir), spinach (Amaranthus spinosus) and cucumber (Cucumis sativus, L). The method used was 0.5 g leaf samples were crushed with 5% metaphosphoric acid. The solution obtained was titrated with dichlorophenol-indophenol (DCIP) 0.8 g / l. Treatment analysis of ascorbic acid (ASA) content in this plant tissue which has the largest ascorbic acid content in cucumber plants (Cucumis sativus L) of 69855.85; then followed by spinach plants (Ipomoea reptana Poir) at 66517.24 and finally in spinach (Amaranthus spinosus) at 38859.13. And the ASA mg standard 308.
References
REFERENSI
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Chaves M. 1991. Effect water deficit on carbon assimilation. J Exp Bot 42:1-6.
Drake BG, Miquel A, Gonzàlez M. 1997. MORE EFFICIENT PLANTS: A Consequence of Rising Atmospheric CO2?. Plant Physiology and Plant Molecular Biology .48:609-639
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Koji S, Winslow RB. Cellular and Subcellular Localization of Phototropin 1. The Plant Cell, 14:1723–1735
Lakitan, B. 1995. Dasar-dasar Fisiologi Tumbuhan. PT. Raja Grafindo Persada, Jakarta.
Lawlor DW. 2002. Limitation to photosynthesis in water-stressed leaves: stomata vs metabolism and the role of ATP. Ann Bot 89:871-885.
Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidant defense system, pigment composition and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol. 119:1091-1099.
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May Jm. 1999. Is ascorbic asid an antioxidant for plasma membrane?. Faseb Journal 13:995-1006.
McKersie BD, Leshem YY. 1994. Stress and Stress Coping in Cultivated Plants. Netherlands: Kluwer Academic Publishers.
Meyer S, Genty B. 1998. Mapping intercellular CO2 mole fraction (Ci) in Rosa rubiginosa leaves fed with abscisic acid by using chlorophyll fluorescence imaging: significance of Ci estimated from leaf gas exchange. Plant Physiol 116:947–957.
Miyao M. 2002. Molecular evolution and genetic engineering of C4 photosynthetic enzymes. J Exp Bot. 54: 179-189
Mohammadkhani, N. and R. Heidari. 2008. Water Stress Induced Stomatal Closure in Two Maize Cultivars. R. J. Bio. Sci. 3 (7) : 750 - 754.
Munné-Bosch S, Schwarz K, alegre L. 1999. Enhaced formation of ?-tokoferol and highlyoxidizedabieten diterpenes in waterstressed rosemary plants. Plant Physiol 121:1061-1068.
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O’toole, JC, Garrity DP. 1984. Upland Rice Soil-Plant-Water Relationship. An Overview of Upland Rice Research.International Rice Research Institut. Manila. 1:395-411.
Prohazkova D, Sairam RK, Srivastava GC, Singh DV. 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci 161:765-771.
Roxas, V.P., S.A. Lodhi, D.K. Garrett, J.R. Mahan and R.D. Allen, 2000. Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase. Plant Cell Physiol., 41: 1229-1234
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Schwanz P, Polle A. 2001. Differential stress responses of antioxidative systems to drought in penduculate oak (Quercus robur) and maritime pine (Pine pinaster) grown under high CO2 concentration. J Exp Bot 52(354):133-143.
Smirnoff N. 1996. The function and metabolism of ascorbic acid in plants. [botanical briefing]. Ann Bot 78:661-669.
Sonja D, Veljovic-Jovanovic, Cristina P, Graham N, Christine HF. 2001. Low askorbic acid in the vtc-1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system. Plant Physiol 127:426–435.
Taiz L, Zeiger E. 2002. Plant Physiology. Sunderland: Sinauer Associates. hal 690.
Tausz M, Wonisch A, Peters J, Jimenez MS, Morales D, Grill D.2004. Short-term changes in free-radical scavengers and chloroplast pigments in Pinus canariensis needles as affected by mild drought stress. Journal of Plant Physiology. 158, 213–219.
Tezara W, Mitchell V, Driscoll SP, Lawlor DW. 2002. Effects of water deficit and its interaction with CO2 supply on the biochemistry and physiology of photosynthesis in sunflower. J Exp Bot 53:1781-1791
Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxydative stress, and signal transduction. Plant Biol 55:373-399.
Aroca R, Juan JI, Manuel SD. 2001. Photosynthetic characteristics and protective mechanisms against oxidative stress during chilling and subsequent recovery in two maize varieties differing in chilling sensitivity. Plant Sci 161:719–726.
Becana M, Moran JF, Ormaetxe. 1988. Iron-dependent oxygen free radical generation in plants subjected to environmental stress: toxicity and antioxidant protectio. Plant and Soil. 201: 137–147
Berkowitz GA. 1998. Water and Salt Stress. In: Raghavendra AS (ed). Photosynthesis: A Comprehensive Treatise. Cambridge: Cambridge University Pr; p. 226-237.
Brodribb TJ, Holbrok NM. 2003. Stomatal closure during leaf dehydration, correlation with other leaf physiological traits. Plant Physiol 132:2166-2173.
Chaves M. 1991. Effect water deficit on carbon assimilation. J Exp Bot 42:1-6.
Drake BG, Miquel A, Gonzàlez M. 1997. MORE EFFICIENT PLANTS: A Consequence of Rising Atmospheric CO2?. Plant Physiology and Plant Molecular Biology .48:609-639
Foyer CH, Descourvieres P, Kunert KJ (1994). Photoxidative stress in plants. Plant Physiol. 92: 696-717.
Goldsworthy, P.R. dan N.M. Fisher. 1992. Fisiologi Tanaman Budidaya Tropik (diterjemahkan oleh Tohari). Gajah Mada University Press, Yogyakarta.
Hamim. 1995. Toleransi kedelai terhadap cekaman kekeringan: Pendekatan morfologi dan fisiologi [tesis]. Bogor: Program Pascasarjana, Instutut Pertanian Bogor.
Hamim. 2005. Respon pertumbuhan spesies pertumbuhan spesies C3 dan C4 terhadap cekaman kekeringan dan konsentrasi CO2 tinggi. Biosfera 22:105-113
Islami, T. dan W.H. Utomo. 1995. Hubungan Tanah, Air dan Tanaman. IKIP Semarang Press, Semarang.
Kele? Y, Öncel I. 2002. Response of antioxidative defence system to temperature and water stress combination in wheat seedlings. Plant Sci 163:783-790.
Koji S, Winslow RB. Cellular and Subcellular Localization of Phototropin 1. The Plant Cell, 14:1723–1735
Lakitan, B. 1995. Dasar-dasar Fisiologi Tumbuhan. PT. Raja Grafindo Persada, Jakarta.
Lawlor DW. 2002. Limitation to photosynthesis in water-stressed leaves: stomata vs metabolism and the role of ATP. Ann Bot 89:871-885.
Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F (1999) Antioxidant defense system, pigment composition and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol. 119:1091-1099.
Mathius, N.T., G. Wijana, E. Guharja, H. Aswindinnoor, Y. Sudirman, dan Subronto. 2001. Respon Tanaman Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Cekaman Kekeringan. Menara Perkebunan 69 : 29 - 45.
May Jm. 1999. Is ascorbic asid an antioxidant for plasma membrane?. Faseb Journal 13:995-1006.
McKersie BD, Leshem YY. 1994. Stress and Stress Coping in Cultivated Plants. Netherlands: Kluwer Academic Publishers.
Meyer S, Genty B. 1998. Mapping intercellular CO2 mole fraction (Ci) in Rosa rubiginosa leaves fed with abscisic acid by using chlorophyll fluorescence imaging: significance of Ci estimated from leaf gas exchange. Plant Physiol 116:947–957.
Miyao M. 2002. Molecular evolution and genetic engineering of C4 photosynthetic enzymes. J Exp Bot. 54: 179-189
Mohammadkhani, N. and R. Heidari. 2008. Water Stress Induced Stomatal Closure in Two Maize Cultivars. R. J. Bio. Sci. 3 (7) : 750 - 754.
Munné-Bosch S, Schwarz K, alegre L. 1999. Enhaced formation of ?-tokoferol and highlyoxidizedabieten diterpenes in waterstressed rosemary plants. Plant Physiol 121:1061-1068.
Neumann PM. 2008. Coping mechanisms for crop plants in drought-prone environments. Ann Bot 101:901-907.
Niyogi, K.K. 1999. Photoprotection revisited: Genetic and molecular approaches. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 333-359
Noctor G, Foyer GH. 1998. Ascorbate and glutathione: Keeping active oxygen under control. Plant Physiol 49:249-279.
O’toole, JC, Garrity DP. 1984. Upland Rice Soil-Plant-Water Relationship. An Overview of Upland Rice Research.International Rice Research Institut. Manila. 1:395-411.
Prohazkova D, Sairam RK, Srivastava GC, Singh DV. 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci 161:765-771.
Roxas, V.P., S.A. Lodhi, D.K. Garrett, J.R. Mahan and R.D. Allen, 2000. Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase. Plant Cell Physiol., 41: 1229-1234
Salisbury & Ross, 1992. Plant Physiology. 4th ed. Terjemahan Diah R Lukman & Sumaryono Jilid 2. ITB Bandung.
Schwanz P, Polle A. 2001. Differential stress responses of antioxidative systems to drought in penduculate oak (Quercus robur) and maritime pine (Pine pinaster) grown under high CO2 concentration. J Exp Bot 52(354):133-143.
Smirnoff N. 1996. The function and metabolism of ascorbic acid in plants. [botanical briefing]. Ann Bot 78:661-669.
Sonja D, Veljovic-Jovanovic, Cristina P, Graham N, Christine HF. 2001. Low askorbic acid in the vtc-1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system. Plant Physiol 127:426–435.
Taiz L, Zeiger E. 2002. Plant Physiology. Sunderland: Sinauer Associates. hal 690.
Tausz M, Wonisch A, Peters J, Jimenez MS, Morales D, Grill D.2004. Short-term changes in free-radical scavengers and chloroplast pigments in Pinus canariensis needles as affected by mild drought stress. Journal of Plant Physiology. 158, 213–219.
Tezara W, Mitchell V, Driscoll SP, Lawlor DW. 2002. Effects of water deficit and its interaction with CO2 supply on the biochemistry and physiology of photosynthesis in sunflower. J Exp Bot 53:1781-1791
